Karen A. Beitler
Overall Unit Objectives
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1 Review how information is passed from parent to offspring; DNA,--->protein
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2 Define genetic engineering.
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3 Explain how restriction enzymes can be used to make recombinant DNA.
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4 List the steps in a gene-transfer experiment.
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5 Distinguish between the following laboratory techniques: RFLP analysis, gel electrophoresis and polymerase chain reaction.
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6 Explain how DNA technology can be used to produce food & medicine.
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7 Describe some ways that DNA technology can be used to improve crops.
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8 Discuss some environmental and ethical issues in genetic engineering
LP 1
- DNA & Genes - what are they, how we use them, how can they be influenced?
LP 2
- Genetic engineering -RFLP, PCR & DNA fingerprinting
LP 3
-Transferring genes - pGLO lab
LP 4
- Environmental & Ethical Issues in Genetics
Lesson plans may take more than one day. Lessons are presented in an order the writer believes will be most beneficial to the student. Depending on your student population and available time, additional depth and research may be explored.
LP 1
Topic: DNA & Genes - what are they, how we use them, how can they be influenced?
Objectives
The student will. . .
Explain the principal function of DNA
Describe the structure of DNA
Summarize the main features of DNA replication.
Explain the primary function of RNA.
Summarize the process of transcription and translation.
Distinguish between a codon and an anticodon, and state where each is found.
Lesson 1
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1. Lecture & Discussion: What is DNA? How does it reproduce?
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2.
DNA Twizzlers Lab
( Appendix B)
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3. DNA Webquest (
Appendix C)
Lesson 2
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1. Lecture & Discussion: DNA replication; processes & function
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2. DNA- The Double Helix (see Student resource 13)
LP 2
Topic: Genetic engineering - PCR & DNA fingerprinting
Objectives
The student will. . .
Explain the primary functions of DNA and RNA.
Summarize the process of transcription and translation.
Define genetic engineering.
Explain how restriction enzymes can be used to make recombinant DNA.
Explain how cloning vectors can be used to clone and transfer genes.
Lesson 1
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1. Lecture & Discussion: What is genetic engineering?
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2.
Nucleus to Fingerprint
( Trace the life of a gene in a poster)
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3. Resource
: Lifeedu.org
Lesson 2
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1. Lecture & Discussion: What is PCR?
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2. Practice PCR in an online tutorial at the website called Polymerase Chain Reaction
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(Cold Spring Harbor, 1)
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3. View the Principle of the PCR at
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http://users.ugent.be/~avierstr/principles/pcrani.html
Lesson 3
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1. Lecture & Discussion: Methods in Genetic engineering
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2.
Learn Genetics
website practice
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3.
Gel electrophoresis
- run standards and DNA fragments
or
practice online
LP 3
Topic- Genetic Transformation
Objectives
The student will. . .
Explain how gene therapy may be used in humans
Explain how DNA technology can be used to produce medical products.
Lesson 1
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1. Lecture & Discussion: What is RFLP?
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2.
Paper Genetics lab
-email me for a copy
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3. Or clone a mouse
at http://gslc.genetics.utah.edu/
Lesson 2
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1. Prelab discussion: Bacterial transformation (see
pGLO
kit instructions)
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2.
pGLO
lab from BioRad
or
similar tranformation lab
LP 4
Topic- Environmental & Ethical Issues in Genetics
Objectives
The student will. . .
Discuss environmental and ethical issues in genetic engineering.
Describe some ways that DNA technology can be used to improve crop yields and
the food supply.
Lesson 1
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1. Lecture & Discussion: Ethical Issues in Genetic modification
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2. Student Webquest of Ethical Issues
Lesson 2
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1. Lecture & Discussion: Environmental Issues in Genetic modification
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2. Student research essay Environmental Issues
Lesson 3
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1. Lecture & discussion; How to Persuade
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2. State wide STS Activity; GM Food Persuasive Pamphlet
Lesson 4 -
optional addition
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1. Lecture & Discussion: Biomedical Engineering
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2. PowerPoint presentation -
Careers in Biomedical Engineering
(Farrell, 1)
Lesson 5 -
optional addition
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1.
Designing a Career in Biomedical Engineering
(EMB 2003)
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2. Student research; Careers in Biotechnology
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3. Student presentations; Careers in Biotechnology
